Protocols for Gene Transfer in Neuroscience: Towards Gene Therapy of Neurological Disorders
Protocols for Gene Transfer in Neuroscience: Towards Gene Therapy of Neurological Disorders Books
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Protocols for Gene Transfer in Neuroscience Towards Gene Therapy of Neurological Disorders Edited by P. R. Lowenstein University of Manchester, UK and L. W. Enquist Princeton University, USA The brain and the cells that compose it have everlastingly presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. But, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first matter-of-fact guide to gene delivery, providing over 150 simple to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to know, adapt and expand the technology as needed. Written by a team of international expert neuroscientists, geneticists, molecular biologists and biochemists, Protocols for Gene Transfer in Neuroscience describes the latest techniques from the top gene-delivery labs, ranging from corporal transfection techniques, through the use of a wide range of viral vectors, to retrovirally transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and thwart-referenced so that an investigator can find all the details required for a particular problem.
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