Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology
Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology Books
Product Description
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells.
Patch Clamping aims to cover the basic principles and matter-of-fact applications of this vital technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of consequences.
- This book will clarify the basic principles and matter-of-fact application of patch clamp electrophysiology.
- Written in a non-technical style to make sure its broad fascinate to novice users
- Takes a matter-of-fact approach
- This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
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I agree with the other reviewers on the top that this is the only book available on this theme, because this is a real desert, but that is not just so right, you can get detailed in rank about how to do patch clamp by looking at Current protocols in neuroscience for example, but that is a chapter only in a book.
The book is detailed in the sense that it tells you what equipment you need etc if you are setting up your own lab or setting up a setup in a lab, but it is terrible in terms of concepts and explanation of them. It gives the impression the author does not know what is he talking about.
I can’t urge you buy this, I suggest that, if you really need to look at it, to get it via interlibrary loan or so, if you can, and see what I am talking about.
Rating: 2 / 5
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I’m giving this book a high rating because it fills a niche for which no other text exists: An absolute beginner’s guide to patch-clamping. Yes, you may possibly read Neher & Sakmann’s hefty “Single Channel Recording” but, for the complete novice patch-clamper, it’s a bit overwhelming.
So, you walk into a patch-clamp lab, there’s a million different things on the rig and you’re feeling very confused. The microscope is much more complex than the microscope you remember from high school, there’s cabling literally the world over, and everybody keeps mentioning how vital it is not to disturb the “voodoo” shielding.
Where do you start? What do all those instruments do? Will I ever patch a cell on my own? This is the book for you.
The book does an brilliant job explaining the main components on a patch-clamp rig (and advice for setting up your own if you’re just starting up a lab) and gives detailed instructions on whole-cell patch clamp techniques. (I have not evaluated the single-channel techniques but they too seem quite well written.) Also, many simple “equivalent circuit” diagrams are given so that you can know what you’re measuring. You will patch successfully after reading this book!
My only criticism is that the book is a bit skimpy on what to do after you’ve got the cell patched. Only a few voltage-clamp techniques are explored and there is very small on experiments using dual recordings.
Otherwise, it’s an brilliant book. If you’re new to patch-clamping, this is (literally) the only book for you.
Rating: 5 / 5
Simply place, this is the best (and in my search, the only) introductory patch clamp guide available. I sought out this book after reading several patch clamp guides that offered small or no help in the fundamental understanding of patch clamp methods. Books such as “Introduction to Electrophysiological Methods and Instrumentation”, “The Axon Guide”, and “Patch-Clamp Analysis: Advanced Techniques” are beyond the scope of the beginner electrophysiologist and are written for researchers with some electrophysiology experience, and with a heavy electrical engineering slant. Others such as “Patch-Clamp Methods and Protocols” are just that… a run of protocols that read as if they are taken directly out of a methods journal. Again, this is fantastic if one is already involved patch clamp and is looking to expand one’s skill set. So after reading all four of these books, I still felt fundamentally frustrated with the explanations of the different methods and still recognized a general lack of fundamental in rank presented in a simplified perspective. I plotting I would give “Patch Clamping: An introductory guide to Patch Clamp Electrophysiology” a last opportunity effort before giving up on finding a “excellent” patch clamp book. It paid off. This is quite simply the best intro to patch clamp I have read… and probably the only one now available. If there is another excellent intro to patch clamp book out there, I was unable to find it. This book is a “must have” to all who are just starting out in patch clamp. I might also mention that “Patch clamp analysis: advanced techniques” is also not a terrible book to have in the lab, but should be read AFTER reading “Patch Clamping” AND after several months of hands on patch clamp experience. Highly Not compulsory!
Rating: 5 / 5
I’m giving this book a high rating because it fills a niche for which no other text exists: An absolute beginner’s guide to patch-clamping. Yes, you may possibly read Neher & Sakmann’s hefty “Single Channel Recording” but, for the complete novice patch-clamper, it’s a bit overwhelming.
So, you walk into a patch-clamp lab, there’s a million different things on the rig and you’re feeling very confused. The microscope is much more complex than the microscope you remember from high school, there’s cabling literally the world over, and everybody keeps mentioning how vital it is not to disturb the “voodoo” shielding.
Where do you start? What do all those instruments do? Will I ever patch a cell on my own? This is the book for you.
The book does an brilliant job explaining the main components on a patch-clamp rig (and advice for setting up your own if you’re just starting up a lab) and gives detailed instructions on whole-cell patch clamp techniques. (I have not evaluated the single-channel techniques but they too seem quite well written.) Also, many simple “equivalent circuit” diagrams are given so that you can know what you’re measuring. You will patch successfully after reading this book!
My only criticism is that the book is a bit skimpy on what to do after you’ve got the cell patched. Only a few voltage-clamp techniques are explored and there is very small on experiments using dual recordings.
Otherwise, it’s an brilliant book. If you’re new to patch-clamping, this is (literally) the only book for you.
Rating: 5 / 5