Metastasis Research Protocols, Volume II: Analysis of Cell Behavior In Vitro and in Vivo
Metastasis Research Protocols, Volume II: Analysis of Cell Behavior In Vitro and in Vivo Books
Product Description
Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of customary and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. Volume I focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The habitual methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, differential show. Volume II presents a comprehensive collection of customary and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, by the side of with assay systems to test defined aspects of the metastatic cataract. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.
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