Lentivirus Gene Engineering Protocols
Lentivirus Gene Engineering Protocols Books
Product Description
Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and matter-of-fact aspects of lentivirus vector-based gene transfer. The authors exhibit lentivirus vector production with methods for recovering apt producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal tint. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.
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