Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale
Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Administer Rationale Books
Product Description
On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Administer Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Calculated for advanced undergraduate and beginning graduate students in molecular biology, this unique combination address/laboratory resource presents detailed protocols for the multi-step administer caught up in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein’s basic corporal properties. This manageable volume includes both theoretical background and matter-of-fact procedures and is structured nearly twenty experiments that exhibit how to prepare, manipulate, and question plasmids, yield fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches calculated to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, even as also giving students the tools needed to become “capable proactive researchers.” Features:
· Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques
· Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students reckon about the precise consequences of solution and procedural manipulations
· Discusses fluorophores, and solvent effects on protein structure
· Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature
· Includes carefully selected primary source research literature and articles from current vendor literature
· Contains a glossary of unfamiliar phrases and jargon; vital summary statements and conclusions are italicized
· Provides an alphabetized list of common reagents for rapid reference
· Offers an extensive index of concepts and terms
· Categorizes helpful and distinctive in rank into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Administer Rationale, Vendor Literature, and Alternative Approaches
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