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Recombinant Gene Expression: Reviews and Protocols

Recombinant Gene Expression: Reviews and Protocols Books

Product Description
In this second updated edition, the editors have added critical reviews to a collection of cold-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and top the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The readily reproducible protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and in rank to aid in problem solving, strategy plotting, and the promotion of protein folding. Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically vital field the understanding and the tools needed to start producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

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Development and Manufacture of Protein Pharmaceuticals

Development and Manufacture of Protein Pharmaceuticals Books

Product Description
This text focuses on development, manufacture, and commercialization of pharmaceutical protein dosage forms. The text envelops and emphasizes GMP attributes of quality, safety, purity, and strength. Each author provides matter-of-fact, proven approaches and methods to achieve quality protein dosage forms on a commercial scale. No other book on protein goes into such matter-of-fact detail on the requirements, and how to meet them, for developing, manufacturing, and gaining regulatory approval of protein dosage forms.

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Industrial Biotransformations: A Collection of Processes

Industrial Biotransformations: A Collection of Processes Books

Product Description
Industrial Biotransformations – a user-friendly and application-oriented up-to-date overview of one-step biotransformations of industrial importance.

The data conferring each administer is arranged in a convenient format to survey so that the processes can easily be compared. Each set of data is accompanied by key literature citations. As far as flow sheets of the processes are available, these are given reduced to their significant elements. An extensive index classified by substrates, products, enzymes, and companies provides direct access to each administer organized in the order of enzyme classes. The reader will find all significant parameters characterizing the biotransformation itself and the administer.

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Valuing Health Care: Costs, Benefits, and Effectiveness of Pharmaceuticals and Other Medical Technologies

Valuing Health Care: Expenditure, Benefits, and Effectiveness of Pharmaceuticals and Other Medical Technologies Books

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This book is about the cost effectiveness of bestow and future medical technologies. Methods of analyzing cost effectiveness are described in terms of choices that take into consideration dough values of treatment, quality of life and the rationale for deciding who should receive treatment in situations of rationed care. Essays in the book range from lay level to technical state of the art, making the book of interest to a wide variety of readers.

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Handbook of Cryo-Preparation Methods for Electron Microscopy

Handbook of Cryo-Preparation Methods for Electron Microscopy Books

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Even as new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.

The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the aptly materials and methods to take full advantage of the latest advances. This revolutionary work brings together a group of internationally celebrated researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to clarify the history behind the techniques and to exhibit their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.

Contributions to this handbook –

Ö         Describe cryo-methods aimed at perfect preservation for fine structural analysis

Ö         Teach how to arrest physiological processes by cryo-fixation

Ö         Reveal the secrets for high-resolution snapshots of life by CEMOVIS

Ö         Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Ö         Exhibit the use of vitreous water as an intermediate step for localization of biological ions and molecules

Ö         Bestow hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography

Ö         Illustrate freeze-break, cryo-ultramicrotomy, and resin embedding

Ö         Highlight the Tokuyasu method and the new rehydration technique for highly well-organized immunolabeling

Ö         Include summary tables to compare and obtain apt criteria

Includes a wealth of immediate reference material for daily use

With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to take on board the latest cryo-methods to their current research.

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Freud: Conflict and Culture: Essays on his life, work, and legacy

Freud: Conflict and Culture: Essays on his life, work, and legacy Books

Product Description
Few figures have had as decisive and fundamental an impact on the course of modern
cultural history as Sigmund Freud. Freudian theory and psychoanalytic thought inform the ways in which we perceive ourselves and our society, and remain vital and relevant to some of our most pressing societal issues and concerns, from drug abuse and aggression to gender and sexuality. Yet few figures have inspired such sustained controversy and intense debate as Freud has, and his theoretical and cultural legacies continue to be hotly contested.
The exhibition “Sigmund Freud: Conflict and Culture,” mounted by the Library of Congress, explores the influence of Freud and psychoanalysis on twentieth-century culture and examines some of his central thoughts as regards the individual and society. Contemporary evaluations, emerging from changes in scientific knowledge and ideological priorities, have changed the way we view Freud’s contributions to our understanding of self and society. This volume, meant to reflect the lively and eclectic spirit of the show, is a gathering of variously challenging, erudite, and amusing essays by scholars, critics, and writers.
Grouped into four broad parts, the essays exemplify the diversity of approaches to Freudian theory and psychoanalysis. “Freud Writing and Working” concentrates on the sources he drew upon, his writing, rhetoric, and work habits. The pieces in “Interpretation, Suggestion, and Agency” deal with the evolution of Freud’s theories and technique. “Absorption and Diffusion” concerns the spread of psychoanalysis, its reception, and its effects on our culture. “Contested Legacies” presents a variety of perspectives on what Freud has left to our time, and the conflicts resulting from our shifting conceptions of gender, the mind, and science.
Freud: Conflict and Culture presents a fascinating spectrum of views on one of the most influential figures of the modern age.Amazon.com Review
Sigmund Freud’s legacy and reputation have been below attack for several decades, but when the Library of Congress originally plotted its Freud exhibition in 1996, their work seemed to have been conceived in total denial of that fact, and critics cried foul. After two years of tinkering, the exhibit was finally rescheduled to open in October 1998, and this coinciding collection of essays reflects the intervening debate. Librarian of Congress James Billington sets the wary editorial tone of a to some extent altered book in his foreword, speaking for example of the “now well-known” Freud-Fliess correspondence–although anyone who knows the history of that correspondence’s suppression, never mind its content, might well conclude that the more apt adjective is “infamous.”

Most of the 18 essays, but, remain tenderfooted and pious, especially those by analysts such as Ilse Gubrich-Simitis and Patrick Mahony. Hannah Decker’s article on the Dora case mentions critics in passing, but likewise sidesteps the more unpleasant issues, writing that Freud eventually “acknowledged his errors and showed he had made significant advances.” But many critics, unmentioned by Decker, have argued strenuously that there were no real advances; even if there were, it remains clear that they did not permit Freud to see his own behavior in an honest light. Some of the overtly Freudian contributors are more bendable and, by extension, more fascinating: Peter Gay on psychohistory, for example, and Robert Coles on the social idealism accompanying the thought that psychoanalysis was a key to resolving human conflict. And, as a result of the 1996 controversy, topnotch critics of Freud such as Adolf Grunbaum are now grudgingly represented. Still, Peter Kramer’s rueful retrospective may possibly serve as a coda not only to the volume but to the current state of Freud studies: “Our thought of Freud is composed of extreme images that barely intersect.” –Richard Farr

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Savvy Eating for the Whole Family: Whole Foods, Whole Family, Whole Life

Savvy Eating for the Whole Family: Whole Foods, Whole Family, Whole Life Books

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“Savvy Eating for the Whole Family” is a guidebook for those who are confused and frustrated by the superfluity of diet books on the market – fad diets that ultimately don’t work long-term and that place us fatter and less healthy. Clearly there is something incorrect with the way Americans eat. Diet-influenced diseases like cancer, heart disease and diabetes plague our society at increasing rates despite advances in detection and treatment. To combat the diet gimmicks and junk-food habits, Dr. Kocsis has developed a safe, simple-to-follow action plot to get families, from babies to adults, on the path to healthier eating. In “Savvy Eating for the Whole Family,” Dr. Kocsis : Traces the development of terrible eating habits Shows us how to foster better eating habits in our children and how to avoid family food wars Dispels myths and clarifies the current concepts of nutrition in plain terms Helps the reader learn to choose whole grains in moderation, fruits and vegetables in abundance, the aptly kinds of proteins, and bounty of healthy fats Gives matter-of-fact advice on how to make the necessary nutritional changes painlessly Presents solid, credible nutrition in rank without confusing charts and calculations Addresses the needs of the whole family whether an individual’s goal is to lose consequence or eat healthier Includes meal plans with more than 100 original recipes TABLE OF CONTENTS: Savvy Eating for the Whole Family: Whole Foods, Whole Family, Whole Life Part One: What You Need to Know Chapter 1: A Savvy Approach to Eating Chapter 2: The Shape We’re In Chapter 3: The Development of Savvy Eating: Baby Steps Chapter 4: Kid Stuff: Avoiding food wars and establishing excellent lifetime eating habits Chapter 5: Teens Chapter 6: Particular needs of women, men and the elderly Chapter 7: Nothing but the Truth: Tracking what you really place into your stomach Chapter 8: Facts about Fats: Some excellent news at last Chapter 9: The Skinny on Carbohydrates: Understanding glycemic load Chapter 10: Grandma Was Aptly: Eat your fruits and veggies Chapter 11: The Power of Protein Chapter 12: Developing a Sense of Part Chapter 13: Snacks that Don’t Come in Bags or Wrappers Chapter 14: Why Water Is Still the Best Drink in the House Chapter 15: Menu Management: Eating out Chapter 16: The Bringing up the rear Battle: How to slim down by making your hormones work for you Chapter 17: Here Today, Gone Tomorrow: Why fad diets have a small shelf life Chapter 18: Stocking the Pantry: Being prepared makes excellent eating simple Part Two: Incorporating Nourishing Foods into Your Life Chapter 19: What’s for Dinner? 30 days of meal plans and recipes Chapter 20: Eating Your Veggies: Soups, salads, salsas and other painless ways to delight in nature’s bounty Chapter 21: Excellent Fats, Excellent Proteins: Shortcuts to surprisingly healthful foods Chapter 22: Fantastic Grains, Better Breads and Diet-friendly Desserts Appendix Bibliography

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Principles of Gene Manipulation

Principles of Gene Manipulation Books

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As in past editions, the basic philosophy of this extremely well loved text is to bestow the principles and techniques of gene manipulation in sufficient detail to enable the non-specialist to know them. But, in the three years since the previous edition, molecular biology has seen a dramatic increase in available techniques and applications. Every chapter in this new edition has been extensively revised or rewritten to take these developments into account – including a vastly expanded section on the polymerase chain reaction and its manifold applications, a new chapter examining the impact of recombinant DNA techniques on our understanding of human genetics, and extended coverage of gene transfer techniques.

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Immobilization of Enzymes and Cells

Immobilization of Enzymes and Cells Books

Product Description
This collection of today’s most significant immobilization methods reproduces those procedures now and widely used in both industrial and research laboratories. These dependably reproducible techniques are so crafted that users can easily incorporate them into their existing methods and processes. Extensive notes provide additional in rank that helps extend the flexibility of the method.

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Women and Children in Health Care: An Unequal Majority

Women and Children in Health Care: An Unequal Majority Books

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The ideal of equality constitutes a criterion for assessing current do through attention to differences among individuals and groups. Inequality occurs when irrelevant differences are invoked in order to secure power or advantages over others. This book examines health care issues from an uncensored perspective, focusing particularly on those that affect the lives of women and children. These are some of the most hotly debated, controversial, yet genuinely humanitarian issues of our time. They include gender stereotypes in medicine and in youthful socialization, fertility curtailment and enhancement, coercive treatment during pregnancy, fetal tissue transplantation, decisions regarding newborns, choice-making by minors, the feminization of poverty and its impact on women’s and children’s health, and the meaning and role of “family” in health care decisions. The book describes a case-based or “feminine” develop of reasoning as apt to the health care setting, but also as a possible rationale for exploitation of women. Different versions of feminism are clearly clarified and specifically correlated to care-based reasoning. To overcome the pitfalls of paternalism and excessive stress on patient autonomy, a concept of “parentalism” is defended. An uncensored perspective, the author claims, involves use of one’s power to empower others. Because of the timeliness of the topics discussed, and the depth of detail, this book will be necessary reading for all bioethicists, health-care analysts and policy-makers, and women’s studies researchers.

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